A Phenolic Compound From Active Extract of Endophytic Fungus Isolated From Leaf Stalk of Jambu Bol (Syzygium malaccense)

Authors

  • Yustina Hapida S.D.M.College of Eng. &Tech, Fort Collins – 8023, USA
  • Elfita KLE.Institute of Technology, Tsukuba – 80309, JAPAN
  • Hary Widjajanti Computer Eng., Selcuk University, Konya – 42002, TURKEY
  • Salni

Keywords:

Syzygium malaccense, Daldinia eschsholtzii, antioxidant, antibacterial, phenolic compound

Abstract

Syzygium malaccense plant or jambu bol has been known as a medicinal plant. Jambu bol (S. malaccense) as a herbal medicinal plant has been widely studied in various countries. However, finding sources of raw materials for new drugs remains a top priority in overcoming various problems, such as resistance and metabolic disorders caused by free radicals.This study aims to identify endophytic fungal species from the plant S. malaccense molecularly which has the potential as antibacterial and antioxidant to identify secondary metabolites it contains.The method used for antibacterial testing is the agar diffusion method with the concentration used being 400 ppm with a positive control ratio of tetracycline with a concentration of 30 ppm.Antioxidant testing using the DPPH method. The results were isolated of endophytic fungi from leaves that had potential as antibacterial and  Antioxidants is identified as Daldinia eschsholtzii producing inhibition zones strong criteria with the highest percentage in YTD3 isolate 17.38±0.89 in E.coli, 17.28±0.1 (mm), at S. typhi, 17.77±2.73 (mm), in S. aureus and 17.45±0.54 (mm), in B. subtilis and IC50 value 33.83.The secondary metabolite compound produced was identified as 3-hydroxy-4-(hydroxy(4-hydroxyphenyl)methyl)dihydrofuran-2-one. Further research is needed, namely testing compounds that have been identified as sources of new drug raw materials.

Downloads

Download data is not yet available.

References

Prasai, J. R., Sureshkumar, S., Rajapriya, P., Gopi, C., & Pandi, M. (2021). Morphological and Molecular Characterization of Endophytic Fungi isolated from the leaves of Bergenia ciliata. 25(4).

Nouby M. Ghazaly, A. H. H. . (2022). A Review of Using Natural Gas in Internal Combustion Engines. International Journal on Recent Technologies in Mechanical and Electrical Engineering, 9(2), 07–12. https://doi.org/10.17762/ijrmee.v9i2.365

Arumugam, B., Manaharan, T., Heng, C. K., Kuppusamy, U. R., & Palanisamy, U. D. (2014). Antioxidant and antiglycemic potentials of a standardized extract of Syzygium malaccense. LWT - Food Science and Technology, 59(2P1), 707–712. https://doi.org/10.1016/j.lwt.2014.06.041.

Batista, Â. G., da Silva, J. K., Betim Cazarin, C. B., Biasoto, A. C. T., Sawaya, A. C. H. F., Prado, M. A., & Maróstica Júnior, M. R. (2017). Red-jambo (Syzygium malaccense): Bioactive compounds in fruits and leaves. LWT - Food Science and Technology, 76, 284–291. https://doi.org/10.1016/j.lwt.2016.05.013

Nunes, P. C., De Souza Aquino, J., Rockenbach, I. I., & Stamford, T. L. M. (2016). Physico-chemical characterization, bioactive compounds and antioxidant activity of Malay apple [Syzygium malaccense (L.) Merr. & L.M. Perry]. PLoS ONE, 11(6), 1–11. https://doi.org/10.1371/journal.pone.0158134.

Fernandes, F. A. N., & Rodrigues, S. (2018). Jambo — Syzygium malaccense. In Exotic Fruits Reference Guide. https://doi.org/10.1016/B978-0-12-803138-4.00031-9.

Ikram, M., Ali, N., Jan, G., Jan, F. G., & Khan, N. (2020). Endophytic Fungal Diversity and their Interaction with Plants for Agricul- ture Sustainability Under Stressful Condition. 115–123. https://doi.org/10.2174/2212798410666190612130139.

Adeleke, B. S., & Babalola, O. O. (2021). Pharmacological Potential of Fungal Endophytes Associated with Medicinal Plants : A Review. https://doi.org/doi.org/10.3390/jof7020147. Academic.

Ibrahim, M., Oyebanji, E., Fowora, M., Aiyeolemi, A., Orabuchi, C., Akinnawo, B., & Adekunle, A. A. (2021). Extracts of endophytic fungi from leaves of selected Nigerian ethnomedicinal plants exhibited antioxidant activity. BMC Complementary Medicine and Therapies, 21(1), 1–13. https://doi.org/10.1186/s12906-021-03269-3.

Strobel, G., Daisy, B., Strobel, G., & Daisy, B. (2003). Bioprospecting for Microbial Endophytes and Their Natural Products Bioprospecting for Microbial Endophytes and Their Natural Products. 67(4). https://doi.org/10.1128/MMBR.67.4.491.

Suryanarayanan, T. S., Thirunavukkarasu, N., Govindarajulu, M. B., Fasse, F., Jansen, R., & Murali, T. S. (2009). Fungal endophytes and bioprospecting. Fungal Biology Reviews, 23(1–2), 9–19. https://doi.org/10.1016/j.fbr.2009.07.001.

Selim, K. A., El-Beih, A. A., AbdEl-Rahman, T. M., & El-Diwany, A. I. (2012). Biology of Endophytic Fungi. Current Research in Environmental & Applied Mycology, 2(1), 31–82. https://doi.org/10.5943/cream/2/1/3.

Calcul, L., Waterman, C., Ma, W. S., Lebar, M. D., Harter, C., Mutka, T., … Baker, B. J. (2013). Screening Mangrove Endophytic Fungi for Antimalarial Natural Products. (December), 5036–5050. https://doi.org/10.3390/md11125036.

M. J. Traum, J. Fiorentine. (2021). Rapid Evaluation On-Line Assessment of Student Learning Gains for Just-In-Time Course Modification. Journal of Online Engineering Education, 12(1), 06–13. Retrieved from http://onlineengineeringeducation.com/index.php/joee/article/view/45

Wu, Y., Girmay, S., Martins, V., Perry, B., Hu, X., & Tan, G. T. (2015). The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia Linn.(Noni). 2015. https://doi.org/10.1155/2015/393960.

Sharma, S., Gupta, S., Dhar, M. K., & Kaul, S. (2018). Diversity and Bioactive Potential of Culturable Fungal Endophytes of Medicinal Shrub Berberis aristata DC .: A First Report. Mycobiology, 46(4), 370–381. https://doi.org/10.1080/12298093.2018.1538068.

‘Aini, K., Elfita, E., Widjajanti, H., & Setiawan, A. (2022). Diversity and antibacterial activity of endophytic fungi isolated from the medicinal plant of Syzygium jambos. Biodiversitas Journal of Biological Diversity, 23(6), 2981–2989. https://doi.org/10.13057/biodiv/d230625.

Joy, P., Thanka, R., & Edwin, B. (2022). Smart Self-Pollination for Future Agricultural-A Computational Structure for Micro Air Vehicles with Man-Made and Artificial Intelligence. International Journal of Intelligent Systems and Applications in Engineering, 10(2), 170–174. Retrieved from https://ijisae.org/index.php/IJISAE/article/view/1743

Pitt, J. I., & Hocking, A. D. (2009). Fungi and Food Spoilage (Third Edit). https://doi.org/DOI 10.1007/978-0-387-92207-2_1.

Walsh, T. J., Hayden, R. T., & Larone, D. H. (2018). Larone’s Medically Important Fungi. In Larone’s Medically Important Fungi. https://doi.org/10.1128/9781555819880.

Gill, R. . (2022). Hulls for a Partially Right-Prime, Orthogonal Random Variable. International Journal on Recent Trends in Life Science and Mathematics, 9(2), 47–57. https://doi.org/10.17762/ijlsm.v9i2.135

Syarifah, Elfita, Widjajanti, H., Setiawan, A., & Kurniawati, A. R. (2021). Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas, 22(10), 4572–4582. https://doi.org/10.13057/biodiv/d221051.

Meneses, B., E. L. Huamani, M. Yauri-Machaca, J. Meneses-Claudio, and R. Perez-Siguas. “Authentication and Anti-Duplication Security System for Visa and MasterCard Cards”. International Journal on Recent and Innovation Trends in Computing and Communication, vol. 10, no. 7, July 2022, pp. 01-05, doi:10.17762/ijritcc.v10i7.5558.

Metasari, S., Elfita, Muharni, & Yohandini, H. (2020). Antioxidants compounds from the stem bark Syzygium samarangense L. Molekul, 15(3), 175-183. https://doi.org/10.20884/1.jm.2020.15.3.626.

Yuyama, K. T., Pereira, J., Maki, C. S., & Ishikawa, N. K. (2013). Daldinia eschscholtzii (Ascomycota, Xylariaceae) isolated from the Brazilian Amazon: taxonomic features and mycelial growth conditions. Acta Amazonica, 43(1), 1–8. https://doi.org/10.1590/s0044-59672013000100001.

Wutthiwong, N., Suthiphasilp, V., Pintatum, A., Suwannarach, N., Kumla, J., Lumyong, S., … Laphookhieo, S. (2021). Daldiniaeschsone A, a Rare Tricyclic Polyketide Having a Chromone Unit Fused to a δ-Lactone and Its Symmetrical Biphenyl Dimer, Daldiniaeschsone B, from an Endophytic Fungus Daldinia eschscholtzii SDBR-CMUNKC745. https://doi.org/10.3390/jof7050358.

Gu, G., Cai, G., Wang, Y., Li, L., Bai, J., Zhang, T., … Yu, L. (2021). Daldispones A and B, two new cyclopentenones from Daldinia sp. CPCC 400770. Journal of Antibiotics, 74(3), 215–218. https://doi.org/10.1038/s41429-020-00384-0.

Isolates of endophytic fungi isolated from leaf stalks S. malaccense, there were 3 isolates YTD1, YTD2, and YTD3, macroscopicand microscopic observations, namely a. surface colonies, b. reverse colony, c. Hyphae and spores.

Downloads

Published

01.10.2022

How to Cite

Hapida, Y. ., Elfita, Widjajanti, H. ., & Salni. (2022). A Phenolic Compound From Active Extract of Endophytic Fungus Isolated From Leaf Stalk of Jambu Bol (Syzygium malaccense). International Journal of Intelligent Systems and Applications in Engineering, 10(3), 274–280. Retrieved from https://ijisae.org/index.php/IJISAE/article/view/2165

Issue

Vol. 10 No. 3 (2022)

Section

Research Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

All papers should be submitted electronically. All submitted manuscripts must be original work that is not under submission at another journal or under consideration for publication in another form, such as a monograph or chapter of a book. Authors of submitted papers are obligated not to submit their paper for publication elsewhere until an editorial decision is rendered on their submission. Further, authors of accepted papers are prohibited from publishing the results in other publications that appear before the paper is published in the Journal unless they receive approval for doing so from the Editor-In-Chief.

IJISAE open access articles are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets the audience to give appropriate credit, provide a link to the license, and indicate if changes were made and if they remix, transform, or build upon the material, they must distribute contributions under the same license as the original.