A Comprehensive Study of Solvent Effects on Dipole Moment, Quenching, and Lifetime in Fluorescent Dyes

Authors

  • Manjula Katageri

Keywords:

fluorescent dyes, fluorescence spectroscopy, UV-Vis absorption spectroscopy, non-polar solvents, time-correlated single-photon counting

Abstract

This study examines the influence of solvent polarity and hydrogen bonding on the dipole moment, fluorescence quenching, and fluorescence lifetime of specific fluorescent dyes. We assessed fluorescence intensity and lifetime utilizing a variety of solvents—encompassing non-polar (hexane, toluene), polar aprotic (acetonitrile, DMSO), and polar protic (water, methanol, ethanol, isopropanol)—through UV-Vis absorption spectroscopy, fluorescence spectroscopy, and time-correlated single-photon counting (TCSPC). Our findings indicate that non-polar solvents augment fluorescence intensity and prolong fluorescence lifespan, whereas polar solvents markedly diminish both due to heightened quenching and non-radiative decay. Protic solvents, especially water, have the most pronounced quenching effects owing to hydrogen bonding interactions. The results correspond with the Lippert-Mataga connection, demonstrating that solvent polarity directly influences the excited-state characteristics of fluorescent dyes. This study offers critical insights for enhancing fluorescent dyes applicable in bioimaging, chemical sensing, and materials science.

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References

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Published

28.11.2024

How to Cite

Manjula Katageri. (2024). A Comprehensive Study of Solvent Effects on Dipole Moment, Quenching, and Lifetime in Fluorescent Dyes. International Journal of Intelligent Systems and Applications in Engineering, 12(23s), 2382–2390. Retrieved from https://ijisae.org/index.php/IJISAE/article/view/7347

Issue

Vol. 12 No. 23s (2024)

Section

Research Article

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